Biosynthesis of retinal in bovine corpus luteum.
نویسندگان
چکیده
Bovine corpus luteum tissue was sliced and incubated with beta-[15,15'-(3)H]carotene. The conversion of radioactive beta-carotene into radioactive retinal was substantiated utilizing column chromatography, thin-layer chromatography, high-speed liquid chromatography, and a derivative formation. Lowering of the incubation temperature to 20 degrees C or boiling the tissue eliminated the conversion of beta-carotene to retinal. In addition, other carotenoids and possible oxidation products of beta-carotene in the corpus luteum were investigated. Our results indicate that the bovine corpus luteum possesses the ability to synthesize retinal in situ, which may play a role in reproductive functions.
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ورودعنوان ژورنال:
- Journal of lipid research
دوره 15 4 شماره
صفحات -
تاریخ انتشار 1974